# Scheme release: artic-sars-cov2/400/v5.4.2

**URL:** <https://community.artic.network/t/scheme-release-artic-sars-cov2-400-v5-4-2/546>\
**Category:** Laboratory\
**Created:** [5 August 2024 17:24 UTC](https://community.artic.network/t/scheme-release-artic-sars-cov2-400-v5-4-2/546 "2024-08-05T17:24:15Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![chriken](https://avatars.discourse-cdn.com/v4/letter/c/f9ae1b/32.png) [@chriken](https://community.artic.network/u/chriken)\
**Post date:** [5 August 2024 17:24 UTC](https://community.artic.network/t/scheme-release-artic-sars-cov2-400-v5-4-2/546/1 "2024-08-05T17:24:15Z")

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## Summary
Our collaborators at the British Columba Centre for Disease Control (BCCDC), have updated `artic-sars-cov2/400/v5.3.2` scheme to handle mutations found in the currently dominate lineages (`JN.1`).
## Spike in primers

| chrom | start | end | primername | pool | strand | seq | Spike in uL\* |
| --- | --- | --- | --- | --- | --- | --- | --- |
| MN908947.3 | 3560 | 3584 | SARS-CoV-2\_11\_RIGHT\_1 | 1 | - | CGCTTAAAACACAACTACCGCCCA | 1.82 ul |
| MN908947.3 | 21696 | 21722 | SARS-CoV-2\_69\_RIGHT\_1 | 1 | - | AGTCATGAGTTGAATGTAAAACTGAG | 5.08 ul |
| MN908947.3 | 21696 | 21722 | SARS-CoV-2\_69\_RIGHT\_2 | 1 | - | AGTCCTGAGTTAAATGTAAAACTGAG | 5.08 ul |
| MN908947.3 | 21927 | 21960 | SARS-CoV-2\_70\_RIGHT\_1 | 2 | - | AATTCACAGACTTTAATAAAAACATTAGTAGCG | 3.56 ul |
| MN908947.3 | 22742 | 22774 | SARS-CoV-2\_74\_LEFT\_1 | 2 | + | ATGTCTATGCAGATTCATTTGTAATTAAAGGT | 1.78 ul |

/\* Add X ul of the 100uM primer stock into 200ul of `artic-sars-cov2/400/v5.3.2` 100 uM stock

## Performance improvements

 ![Screenshot 2024-08-05 at 18.23.40](https://europe1.discourse-cdn.com/flex013/uploads/artic/original/1X/e48b76a6bc5ca4abcfa5daa899a22beb2d63a593.jpeg)