Dropout of amplicon 64

Laboratory
#1

Anyone seeing dropout of amplicon 64? Have you been able to fix it?
TIA

#2

We’ve tried dropping the PCR temperature a little (approx 2.5 degrees) and have seen recovery of that amplicon without any other negative effects.

#5

As mentioned above, the amplicon 64 drops out at high temperature above 65 C.
Probably your thermal cycler generates bit higher temperature than the set value.

temparatuer
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