Summary
Our collaborators at the British Columba Centre for Disease Control (BCCDC), have updatedartic-sars-cov2/400/v5.3.2 scheme to handle mutations found in the currently dominate lineages (JN.1).
Spike in primers
| chrom | start | end | primername | pool | strand | seq | Spike in uL* |
|---|---|---|---|---|---|---|---|
| MN908947.3 | 3560 | 3584 | SARS-CoV-2_11_RIGHT_1 | 1 | - | CGCTTAAAACACAACTACCGCCCA | 1.82 ul |
| MN908947.3 | 21696 | 21722 | SARS-CoV-2_69_RIGHT_1 | 1 | - | AGTCATGAGTTGAATGTAAAACTGAG | 5.08 ul |
| MN908947.3 | 21696 | 21722 | SARS-CoV-2_69_RIGHT_2 | 1 | - | AGTCCTGAGTTAAATGTAAAACTGAG | 5.08 ul |
| MN908947.3 | 21927 | 21960 | SARS-CoV-2_70_RIGHT_1 | 2 | - | AATTCACAGACTTTAATAAAAACATTAGTAGCG | 3.56 ul |
| MN908947.3 | 22742 | 22774 | SARS-CoV-2_74_LEFT_1 | 2 | + | ATGTCTATGCAGATTCATTTGTAATTAAAGGT | 1.78 ul |
/* Add X ul of the 100uM primer stock into 200ul of artic-sars-cov2/400/v5.3.2 100 uM stock
