Multiplexing samples


May you, please, let me know how many samples you multiplex per run and how long does it take? I have read 7 samples in 7 hours but also 24 samples in 8 hours, so I am confused.


We have done up to 10 but, in terms of data, could easily do up to 24. The 10, from purified RNA to flow cell running, took us about 12 hours. Could be speeded up by a couple of hours with practice. 24 would take a little longer, particularly in the first wash step.

Hi Helena!

Thank you for your help.
Perhaps we can prepare batches of samples, freeze the libraries and sequence 24.